Agrobacterium-mediated Transformation of Nicotiana tabacum cv. Xanthi Leaf Explants
نویسندگان
چکیده
منابع مشابه
High-Efficiency Agrobacterium-Mediated Transformation of Tobacco (Nicotiana tabacum)
To improve Agrobacterium-mediated transformation of tobacco, factors influencing gene delivery, including genotype of the plant, bacterial strain, and Agrobacterium transformation procedure, were tested via direct somatic embryogenesis. Leaf tissue of three different tobacco genotypes (Nicotiana tabacum L. cvs. Samsun, and Xanthi, and N. benthamiana) we...
متن کاملCloning, Transformation and Expression of Human Interferon α2b Gene in Tobacco Plant (Nicotiana tabacum cv. xanthi)
BACKGROUND Molecular farming is the production of important recombinant proteins in transgenic organisms on an agricultural scale. Interferons are proteins with antiviral and antitumor activities and can be used for viral infections and cancers treatments. OBJECTIVES This study reports the transformation of INF α2b gene in tobacco plant for the first time in Iran. MATERIALS AND METHODS Inte...
متن کاملNucleotide Sequence of the Nicotiana tabacum cv Xanthi Gene Encoding 1-Aminocyclopropane-1-Carboxylate Synthase.
Ethylene is a plant hormone that influences many aspects of plant growth and development (10). The biosynthetic pathway of ethylene production in higher plants is well defined. The rate-limiting step in this pathway is the reaction catalyzed by ACC' synthase (S-adenosyl-L-methionine methyl-thioadenosine-lyase, EC 4.4.1.4), which converts Sadenosyl-L-methionine to ACC. Tobacco (Nicotiana tabacum...
متن کاملcloning, transformation and expression of human interferon α2b gene in tobacco plant (nicotiana tabacum cv. xanthi)
background molecular farming is the production of important recombinant proteins in transgenic organisms on an agricultural scale. interferons are proteins with antiviral and antitumor activities and can be used for viral infections and cancers treatments. objectives this study reports the transformation of inf α2b gene in tobacco plant for the first time in iran. materials and methods interfer...
متن کاملEnhancement of Agrobacterium-mediated transformation efficiency in immature embryo of Triticum aestivum, cv. Arya
An efficient Agrobacterium-mediate transformation method was developed by employing different duration of sonication, Agrobacterium strains, type of inoculation medium and concentrations of acetosyringone in Arya cultivar of wheat. Immature embryos were used as an explant for inoculation with Agrobacterium tumefaciens harboring the recombinant pBI121 plasmid. Among the durations of sonication t...
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ژورنال
عنوان ژورنال: BIO-PROTOCOL
سال: 2019
ISSN: 2331-8325
DOI: 10.21769/bioprotoc.3150